| abstract |
The present invention discloses methods for producing conditionally active proteins that target senescent cells and are conditionally active in the extracellular environment of senescent cells. The method includes discovery methods using libraries of evolved proteins and testing using physiological concentrations of body fluid components. The present invention also discloses conditionally active proteins for killing or removing senescent cells, pharmaceutical compositions using these conditionally active proteins, and methods of treating age-related diseases, conditions or disorders using the pharmaceutical compositions. Conditionally active proteins can be further evolved, conjugated to other molecules, masked, and reduced in activity by attaching cleavable moieties. |