http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110373453-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75884dbd8f979f5eff4233697cca62d4 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fecab5c5cef30c41180f69c627faa69d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_917e2cdb54f35f29a811df107ad3f842 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5992c15f8417bd090357a2aca9380874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9af326305b832624b73db80e2c9207e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_628aa9b8664f862d28175b5089b46449 |
| publicationDate | 2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110373453-A |
| titleOfInvention | A detection primer, probe and kit for KRAS gene mutation site |
| abstract | The present invention provides a detection primer, probe and kit for KRAS gene mutation sites. Currently, the KRAS gene mutation detection sites are seven hot mutation points on the 12th and 13th codons of exon 2 of KRAS gene. : G12S, G12R, G12C, G12D, G12A, G12V, G13D, these seven mutations accounted for more than 98.5% of all mutations. The KRAS gene mutation site detection kits on the market all use 7 tubes to detect the 7 hot spot mutation points of KRAS. The detection process is cumbersome and expensive, and the detection sample type is mainly for tissue or blood samples, and cannot detect feces as an inhibitor. A lot of samples. The present invention reduces seven reaction tubes required for detecting seven mutation points to two reaction tubes, has simple operation steps, lower cost and less time consumption. The sensitivity and specificity of the detection are further improved through ingenious primer design. The invention can detect mutations as low as 1% in 10 ng of sample DNA, and is suitable for clinical practical application and popularization. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113219169-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113219169-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112063701-A |
| priorityDate | 2019-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 217.