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filingDate 2019-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3813693f3dacfeae5cbd2842165bb20
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publicationDate 2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110373449-A
titleOfInvention Methods of capturing nucleic acids comprising target oligonucleotide sequences and uses thereof
abstract The present invention provides a method for capturing nucleic acid comprising a target oligonucleotide sequence from a nucleic acid library, comprising: contacting the nucleic acid library with a probe, the nucleic acid library comprising a nucleic acid comprising a target oligonucleotide sequence, the probe comprising a sequence complementary to a target oligonucleotide sequence, wherein the contacting may be performed in a tetramethylammonium chloride (TMAC) based buffer at a temperature of about 60°C to about 70°C, the contacting may be The target oligonucleotide sequence is hybridized to a sequence complementary to the target oligonucleotide sequence, thereby producing a hybridization product; the hybridization product is separated from nucleic acids in the library that do not contain the target oligonucleotide sequence. The present invention also provides compositions for practicing the above methods.
priorityDate 2018-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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