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filingDate 2019-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a76e11ffc46e88237e8280c3c903a21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6d7fc4ec95ae71cabea42720525ec93
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publicationDate 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110361458-A
titleOfInvention A kind of determination method of guanine in ganciclovir
abstract The invention discloses a method for determining the content of guanine in ganciclovir, which belongs to the technical field of drug analysis and detection, and the detection method is high performance liquid chromatography. The phase chromatographic column has good peak shape, and can be completely separated from ganciclovir to facilitate quantitative analysis; the technical scheme of the present invention is characterized in that: n-hexane-isopropanol is used as the mobile phase, and cellulose-tris(4- Tolyl formate) silica gel as the filler, n-hexane-isopropanol (80:20) as the mobile phase, the flow rate is 0.8~1.5ml/min, the column temperature is 25~35℃, the detection wavelength is 252nm, The sample volume was 10 μL, and the isocratic elution was performed. The method of the invention is simple, reliable, high in sensitivity, good in resolution and reproducibility, accurate and reliable in results, and can be used for controlling the content of guanine in ganciclovir.
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priorityDate 2019-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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