http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110358812-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110358812-B |
| titleOfInvention | A primer and detection method for detecting positive clones of gene knockout and recombination in λ-Red recombination system |
| abstract | The invention discloses a primer and a detection method for detecting positive clones of λ-Red recombination system gene knockout and recombination. It belongs to the field of biotechnology engineering. The invention provides a universal detection primer, which is designed by designing a pair of complementary primers at about 1/3 of the tetracycline tet RA gene and using them in combination with the original identification primers for detecting recombination positives, respectively for detecting the following The 3' end sequence and the 5' end sequence of the λ‑Red recombination system gene knockout recombination positive clone where the tetracycline gene tetRA is the replacement gene. Designing the detection primers in this way provides three options for detecting recombinant positive clones, which can completely avoid the difficult problem of judging positive results due to the small difference between the sequence to be knocked out and the tetracycline tetRA gene. Moreover, the use of universal primers to detect recombinant positive clones can more effectively prove the correctness of the insertion position of the tetracycline tetRA gene. |
| priorityDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID54675776 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419533562 |
Total number of triples: 12.