http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110358812-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29cbe62865e3313705e84bb43bde1877 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3c0eb67d61a001234526c6264fdde8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d806ebac1ed23322051f899cd58e0de6 |
| publicationDate | 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110358812-A |
| titleOfInvention | A kind of primer and detection method for detecting positive clones of gene knockout and recombination in λ-Red recombination system |
| abstract | The invention discloses a primer and a detection method for detecting positive clones of gene knockout and recombination in λ-Red recombination system. It belongs to the field of biotechnology engineering. The invention provides a universal detection primer, the design method of which is to design a pair of complementary primers at about 1/3 in the middle of the tetracycline tet RA gene, and use them in combination with the original identification primers for detection of recombination positive, respectively for detection of tet RA. The tetracycline gene tetRA is the 3'-end sequence and 5'-end sequence of the λ-Red recombination system knockout recombination positive clone of the replacement gene. The detection primers are designed in this way, so that there are three options for detecting recombinant positive clones, which can completely avoid the difficulty in judging positive results caused by the small difference between the sequence to be knocked out and the tetracycline tetRA gene. Moreover, the use of universal primers to detect recombination-positive clones can more effectively prove the correctness of the insertion position of the tetracycline tetRA gene. |
| priorityDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.