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filingDate 2019-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110358756-B
titleOfInvention Construction of protein toggle switch and application thereof in fermentation production of xylonic acid by mixed sugar
abstract The invention discloses a construction of a protein toggle switch and application thereof in fermentation production of xylonic acid by mixed sugar, belonging to the technical field of bioengineering. The protein level toggle switch constructed by the invention has the advantages of short response time and high regulation precision because regulation and control occur at the protein level and the principle is cutting and induced degradation triggered by protease. By utilizing the strategy, when the concentration ratio of the glucose to the xylose in the substrate is more than 1, the carbon metabolism repression phenomenon can be effectively relieved. When the ratio of glucose: when the ratio of xylose is 2:1, the introduction of the toggle switch can improve the yield of the xylonic acid by 0.79 time compared with a control group. The tool has good application prospect when cheap cellulose hydrolysate is used as a substrate to produce high value-added chemicals.
priorityDate 2019-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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