http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110349624-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B20-30 |
| filingDate | 2019-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110349624-B |
| titleOfInvention | Method for positioning T-DNA insertion site by sam file flag tag |
| abstract | The invention belongs to the technical field of biology, and discloses a method for rapidly positioning a T-DNA insertion site by a sam file flag tag, which comprises the following steps of preparing a sample; performing whole genome re-sequencing; obtaining clean _ data of the removed joint according to the sequencing result; comparing the sequencing result with the sequence of the transgenic vector; according to the flag tag of the comparison result file, removing the sequences which are completely compared and cannot be compared to the carrier, and simultaneously removing the tags which are compared for many times; extracting readsID and sequence of the screened flag tag value; and (3) carrying out sequence alignment on the obtained sequence and an arabidopsis genome. According to the comparison result, the sequence which can be completely compared to the genome of the arabidopsis thaliana is removed; screening the residual reads sequences to obtain insertion sites; and eliminating false positive values in the insertion sites to obtain final insertion sites. |
| priorityDate | 2019-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID43672 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419535645 |
Total number of triples: 14.