http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110346436-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3277 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3278 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2019-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110346436-B |
| titleOfInvention | Substrate-free electrochemical biosensor based on signal amplification of non-enzymatic nanomaterials for the detection of uracil-DNA glycosylase |
| abstract | The invention relates to a substrate-free electrochemical biosensor based on signal amplification of non-enzymatic nanomaterials for detecting uracil-DNA glycosylase. The non-enzymatic nanomaterial (OAPS-Por) used in the sensor can not only adsorb a large amount of electroactive substance thionine molecules, but also catalyze the reduction of thionine on the electrode without the participation of H 2 O 2 , Greatly enhances the signal strength. On the basis of this material, a signal probe (OAPS‑Por/Thi@AuNPs‑ssDNA) was prepared. In the presence of UDG, the uracil base in the hairpin DNA on the electrode was removed, and the hairpin structure was unfolded. Complementary pairing attaches the signal probe to the electrode surface, resulting in an amplified thionine reduction peak. The constructed biosensor exhibited a wide linear range (0.005‑1 U/mL) and a low detection limit (0.000697 U/mL). In addition, the biosensor can also be used to detect UDG activity inhibitors and UDG activity in HeLa cell lysates, showing great potential in clinical diagnosis and biomedical research. |
| priorityDate | 2019-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.