http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110343677-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y111-01006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-16 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 |
filingDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110343677-B |
titleOfInvention | Method for improving pichia pastoris catalase expression quantity |
abstract | The invention relates to a method for improving the expression level of pichia pastoris catalase, which comprises the following steps: s1, providing a pichia pastoris seed solution, a basic salt culture medium and a fermentation tank; s2, adding the basic salt culture medium into the fermentation tank, and then performing pretreatment and parameter setting on the fermentation tank; s3, inoculating the seed liquid into a basic salt culture medium in the fermentation tank for fed-batch fermentation, and continuously adding glycerol for fermentation culture after the glycerol in the basic salt culture medium is consumed in the fed-batch fermentation process; s4, after the glycerol added in the step S3 is consumed, adding methanol and a culture medium to continue the fed-batch fermentation. The method can obviously improve the expression quantity and the enzyme activity of catalase by optimizing the fermentation process of pichia pastoris. |
priorityDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 189.