http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110343653-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
filingDate | 2019-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110343653-B |
titleOfInvention | Method for improving yield of 1,2,4-butanetriol by knocking out aldehyde dehydrogenase gene of escherichia coli |
abstract | The invention relates to a method for knocking out an aldehyde dehydrogenase gene of escherichia coli to improve the yield of 1,2,4-butanetriol, and belongs to the technical field of genetic engineering. The method for knocking out the aldehyde dehydrogenase genes (feaB, aldB and ydcW) of the 1,2,4-butanetriol escherichia coli byproduct production pathway by using the CRISPR-Cas9 method improves the yield of the 1,2, 4-butanetriol. The knockout bacteria E.coli W1, E.coli W2 and E.coli W3 ferment xylose to synthesize 1,2,4-butanetriol under aerobic conditions, the 3, 4-dihydroxybutyric acid is respectively reduced by 68.13%, 60.94% and 76.56%, the BT yield reaches 16.50g/L, 15.50g/L and 16.00g/L, and the yield of 1,2,4-butanetriol is effectively improved. |
priorityDate | 2019-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 604.