http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110333308-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-045 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 |
| filingDate | 2019-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110333308-B |
| titleOfInvention | Method for simultaneously determining NNAL and cotinine in urine with high sensitivity and accuracy |
| abstract | The invention discloses a method for simultaneously determining NNAL and cotinine in urine with high sensitivity and accuracy. The method comprises the following steps: 1) preparing a single standard stock solution containing NNAL, cotinine, isotope-labeled NNAL and isotope-labeled cotinine; 2) respectively preparing mixed standard sample working solutions containing NNAL and cotinine in series concentration, and preparing a standard curve; 3) taking a urine sample to be detected, adding phosphate buffer solution containing isotope labeled internal label, and adding 15-25 mu L beta-glucuronidase for enzymolysis; 4) taking out the sample liquid after enzymolysis, placing the sample liquid to normal temperature, carrying out solid phase extraction, carrying out instrument analysis, and calculating according to the standard curve prepared in the step 2) to obtain the contents of NNAL and cotinine. The method effectively solves the problems of complex pretreatment and long time consumption of the existing simultaneous testing method for NNAL and cotinine in urine, greatly reduces the matrix effect in urine, and improves the detection sensitivity and accuracy. |
| priorityDate | 2019-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.