| abstract |
The present invention relates to a kind of Biotransfer process for preparing of steroid hormone substance intermediate, belong to biopharmaceutical technology.The present invention first prepares the steroidal suspension of intermediate compound I, then puts into the fermentation medium containing mould seed suspension, C occurs under the action of mould 11 Position hydroxylation reaction, and C is hydrolyzed simultaneously 21 Position acetate, obtains intermediate II.15g/L or more can be improved in the operation that the present invention is fed intake using emulsification feeding method instead of organic solvent dissolution substrate, the concentration for putting into substrate, and biological transformation ratio is increased to 90% or more.It can be had additional nutrients in feed liquid substance using this method, keep bioconversion more thorough in the case where improving substrate feed concentrations, production cost significantly reduces. |