http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110331159-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110331159-B |
| titleOfInvention | Method for directly secreting and expressing mature double-chain insulin glargine by using saccharomyces cerevisiae |
| abstract | The invention discloses a method for directly secreting and expressing mature double-chain insulin glargine by using saccharomyces cerevisiae. The method comprises the steps of firstly constructing a chassis cell over-expressing Kex2, then transferring tandem signal peptide and codon-optimized insulin glargine DNA into the chassis cell over-expressing Kex2 to form a saccharomyces cerevisiae co-expression system, and performing protein expression and purification to obtain insulin glargine. The method greatly reduces the difficulty of the production process of the insulin glargine, simplifies the downstream multi-step complicated processing flow in the traditional process, and directly completes the modification work of the yeast cells in cells. At the same time, the method reduces the purification complexity and allows the mature insulin protein to be directly extracted from the culture medium by one-step ion exchange purification at a relatively low cost. In addition, due to the fact that components such as trypsin are not used, byproducts which are close to 50% in the original process are avoided, the fermentation use efficiency of nutrient substances such as carbon sources in the culture medium is improved, and the method has good popularization and application prospects. |
| priorityDate | 2019-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 156.