http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110320169-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
| filingDate | 2018-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110320169-B |
| titleOfInvention | Colorimetric detection method for oxytetracycline residues in milk |
| abstract | A colorimetric detection method for oxytetracycline residue in milk belongs to the technical field of analytical chemistry. The invention firstly uses oxytetracycline Aptamer (APT) OTC ) With biotin-labeled probe CP OTC Annealing and hybridizing to form double-stranded DNA modified on the surface of streptavidin coupled magnetic beads (SDB); probe SP OTC And HP OTC Modifying the surface of gold nanoparticles (AuNPs). When Oxytetracycline (OTC) is present in the system, the OTC is in contact with APT OTC Specific binding and APT OTC Out of SDB, immediately after CP OTC HP modified with AuNPs OTC Hybridizing to form an SDB-AuNPs system; AuNPs surface modified SP OTC Can catalyze TMB-H after being combined with SAv-HRP 2 O 2 And (3) changing the color of the solution, drawing a standard curve by utilizing the relation between the change of ultraviolet absorbance at 370nm and the gradient of oxytetracycline concentration, and measuring the ultraviolet absorbance at 370nm of the sample to be detected to realize the detection of the concentration of oxytetracycline. The method has the characteristics of high sensitivity, low cost, rapidness, easiness in operation and the like, and can realize the sensitive determination of oxytetracycline in the sample. |
| priorityDate | 2018-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.