| abstract |
The invention relates to a method for analyzing a plurality of droplets (4) and selecting a specific droplet therefrom, comprising the steps of: - providing a plurality of droplets (4), - for a droplet of the plurality of droplets (4), measuring at least two light signals, each light signal representing the spatial distribution of light intensity in the droplet for the relevant wavelength channel, - calculating a plurality of parameters from the light signal, - determining the droplet from the calculated parameters The sorting category, - sorting the droplets according to the sorting category, wherein the plurality of parameters include the coordinates of the maximum value of each optical signal and the co-localization parameter, and the at least two calculation parameters used in the determining step include Colocalization parameters. |