http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110305849-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 |
| filingDate | 2019-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110305849-B |
| titleOfInvention | T cell capable of stably expressing CAR (CAR), preparation method and application thereof |
| abstract | The invention provides a T cell capable of stably expressing CAR and a preparation method and application thereof. The preparation method of the T cell stably expressing the CAR comprises the following steps: the CAR gene targeting the cancer cell surface antigen is introduced into T cells via a non-viral plasmid such that the CAR is stably expressed for more than 3 weeks and the foreign gene is not integrated into the T cell chromosome. The invention provides a non-integrated T cell for stably expressing CAR by a non-viral plasmid and a preparation method thereof for the first time, and the special plasmid can be stably expressed for more than 3 weeks after being introduced into the T cell by an electrotransfer method. The invention can effectively avoid the adverse effect of lentivirus and retrovirus on the genome modification of T cells, and can avoid introducing any lentivirus or retrovirus element into the T cells. |
| priorityDate | 2019-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.