http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110305183-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79db17a47bf5093f9d0685196fbc4774 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54151e7931ccfeeaad5d2582ebf2b3bd |
| publicationDate | 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110305183-A |
| titleOfInvention | A kind of preparation method of membrane protein |
| abstract | The present invention proposes a method for preparing a membrane protein, which specifically includes the following steps: S1. dissolving inclusion bodies with a denaturant to form a membrane protein inclusion body solution; S2. mixing the membrane protein inclusion body solution into a lipid cube sample to form a membrane protein-containing solution Lipid cube sample of inclusion body solution; S3. Add denaturant to lipid cube sample containing membrane protein inclusion body solution to form lipid cube sample with hydration degree of 40%; S4. Add membrane protein to lipid cube sample for elution solution to form lipid cube samples containing refolded membrane proteins. For the first time, the present invention realizes the renaturation of membrane protein inclusion bodies directly in lipid cube samples without introducing any detergent, so that the obtained membrane protein can retain the natural conformation to a greater extent, which will be used for subsequent crystallography and other related research. Provide strong support. |
| priorityDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 368.