Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_809a352ee282202ed0034590e33c0704 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2522-101 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6813 |
filingDate |
2018-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bde016776f41dffdcb7e8689968ee9d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8efeb9969c0446741cace53bea727f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e95ac762ee842c9f16de47aac740ab4 |
publicationDate |
2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-110291208-A |
titleOfInvention |
single-chain binding protein |
abstract |
The present invention relates to the use of a single-stranded DNA binding protein (SSB) that exhibits at least 50% of its maximum ssDNA binding capacity in the presence of 500 mM sodium ions to dehybridize DNA molecules or to prevent hybridization of complementary ssDNA, wherein the SSB comprises: SEQ ID The amino acid sequence of NO: 1 or an amino acid sequence at least 75% identical to SEQ ID NO: 1, or a functional fragment thereof, and wherein the DNA molecule is present in or exposed to a solution containing one or more of: (i) At least 350 mM sodium ions, (ii) at least 50 mM potassium ions, (iii) at least 150 mM magnesium ions, or (iv) at least 200 mM calcium ions. |
priorityDate |
2017-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |