| abstract |
The invention relates to a preparation method of 7-aminocephalosporanic acid. The method comprises: adopting a membrane filtration method to separate and obtain a cephalosporin C filtrate from a fermentation broth; and then using a water-immiscible organic solvent to extract the cephalosporin from the filtrate cephalosporin C to obtain a cephalosporin C extract; then back-extract the cephalosporin C in the extract to the water phase and remove the remaining organic solvent through vacuum distillation to obtain a high-concentration cephalosporin C sodium salt solution ; Finally, cephalosporin C can obtain 7-aminocephalosporanic acid whose quality meets the standard after enzymatic hydrolysis, crystallization, filtration, washing and drying. The present invention adopts the combination of extraction and stripping, greatly reduces the impurity in the cephalosporin C sodium salt aqueous solution, improves the quality of the cephalosporin C sodium salt aqueous solution, thereby is beneficial to obtain high-quality 7-aminocephalosporin by enzymatic hydrolysis Alkanoic acid. |