http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110283798-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y108-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0051 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110283798-B |
| titleOfInvention | Purification method of gene recombinant protein Tat-hMsrA |
| abstract | The invention relates to a purification method of a gene recombinant protein Tat-hMsrA, which comprises the following steps: obtaining an escherichia coli liquid for expressing the fusion protein Trx-Tat-hMsrA; centrifuging the escherichia coli liquid, resuspending the thallus precipitate, ultrasonically crushing, and centrifuging again to obtain an inclusion body precipitate; resuspending the inclusion body precipitate in a washing solution, and shearing by using a high-pressure homogenizer to obtain a crushing solution; wherein the washing solution is a phosphate buffer solution containing guanidine hydrochloride, n-dodecanol sarcosine, cysteine and lysozyme; centrifuging the crushing liquid, removing supernatant, adding enterokinase enzyme digestion buffer solution into the protein precipitate to obtain mixed solution; adding a renaturation solution into the mixed solution, and renaturing to obtain a renaturation mixture; and (3) eluting the renaturation mixture by column chromatography, ultrafiltering, concentrating, freezing and drying, and collecting the target protein Tat-hMsrA to obtain the target protein. The purification method of the gene recombinant protein Tat-hMsrA can obtain Tat-hMsrA with higher activity. |
| priorityDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.