| abstract |
The invention discloses a kind of narrow crown black poplar 11 tissue culture and rapid propagation methods, explant is obtained using No. 11 spring branch Greenhouse Water Cultures of narrow crown black poplar, in the MS culture medium for adding 6-BA 0.5-1.5mg/L, NAA 0.02-0.1mg/L, No. 11 sterile bottle seedlings of narrow crown black poplar are obtained by neomorph mode.Take the inducer blade differentiation in the MS culture medium for adding 6-BA 0.2-0.3mg/L, NAA 0.03-0.07mg/L, GA 0.1mg/L of bottle seedling blade, the evoking adventive bud proliferation in the MS culture medium for adding 6-BA 0.1-0.5mg/L, NAA 0.07-0.35mg/L, GA 0.5-1.0mg/L, no offspring complete the induction of adventitious root in the 1/2MS culture medium for adding NAA 0-0.3mg/L, IBA 0-0.3mg/L. |