http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110269014-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K61-40 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K61-40 |
| filingDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110269014-B |
| titleOfInvention | RNA interference method of urechis unicinctus larvae |
| abstract | The invention provides an RNA interference method of urechis unicinctus larvae, and belongs to the technical field of gene interference. The RNA interference method comprises the following steps: (1) taking a culture water body in a normal breeding state of urechis unicinctus larvae, and filtering seawater in the culture water body by using bolting silk to obtain a concentrated water body with the larva density of 15-60/ml; (2) mixing the concentrated water body with target gene double-stranded RNA to enable the final concentration of the target gene double-stranded RNA to be 0.03-0.3 micromole/liter, and standing for 6-15 hours to obtain an interference water body; (3) adding filtered seawater into the interference water body to the culture water body in the normal state in the step (1), and continuing to raise for more than or equal to 2 days. The RNA interference method provided by the invention can effectively interfere the expression of the target gene of the urechis unicinctus larvae, and is beneficial to the research of the function of the urechis unicinctus gene. |
| priorityDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 14.