http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110261519-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110261519-B |
| titleOfInvention | Liquid chromatography-mass spectrometry analysis method for EGCG |
| abstract | The invention belongs to the technical field of LC-MS (liquid chromatography-mass spectrometry) detection, and particularly discloses an EGCG LC-MS analysis method, which comprises the following steps: s1, collecting a sample to obtain a plasma sample; s2, sample pretreatment: adding internal standard working solution, precipitating protein by acetonitrile, and adding methanol: re-dissolving the supernatant with an aqueous solution (10:90, v: v) to obtain a sample to be detected; s3, LC-MS (liquid chromatography-Mass Spectrometry) determination: ion reaction for quantitative analysis: EGCG is m/z457.1 → m/z169.0 and internal standard is m/z269.0 → m/z 105.9; s4, quantitative analysis: and establishing a standard curve, and calculating the concentration of EGCG in the plasma sample according to a linear regression equation and the peak area obtained by detecting the sample to be detected. The LC-MS analysis method has the advantages of high separation degree, high specificity, high sensitivity, high precision and high reproducibility, can realize rapid and accurate quantitative analysis of EGCG in a plasma sample, and has the lower limit of quantification as low as 50 ng/mL. |
| priorityDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.