http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110261371-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-73 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-73 |
| filingDate | 2019-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110261371-B |
| titleOfInvention | Quality control method of microelement additive |
| abstract | The invention relates to a trace element additiveThe quality control method comprises the following steps: s1, inoculating the small intestine epithelial cells to the upper chamber of the cell culture chamber, adding DMEM complete culture solution to the lower chamber, and culturing until the transmembrane resistance value of the small intestine epithelial cells is 300 omega cm 2 ～600Ω·cm 2 Replacing a DMEM complete culture solution with an HBSS buffer solution, and continuously culturing for 12-24 hours to obtain a small intestine single-layer epithelial cell model; s2, removing the HBSS buffer solution in the cell model, adding a test solution into an upper chamber, adding the HBSS buffer solution into a lower chamber, and culturing for 20-26 hours; wherein the test solution is mainly prepared from HBSS buffer solution and trace element additive; s3, collecting the solutions in the upper chamber and the lower chamber respectively and digesting to obtain an upper chamber digestion solution and a lower chamber digestion solution; measuring the upper digestion solution and the lower digestion solution by adopting an ICP (inductively coupled plasma) technology to obtain the concentrations of trace elements in the upper digestion solution and the lower digestion solution; and obtaining the transport rate of the trace element additive according to the concentration of the trace element. |
| priorityDate | 2019-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 16.