http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110257526-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
filingDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110257526-B |
titleOfInvention | Kit for detecting methylation of GP5 gene 5' end and detection method thereof |
abstract | The invention discloses a kit for detecting methylation at the 5 'end of GP5 gene and a detection method thereof, and the kit comprises a GP5 gene 5' end methylation specific amplification reaction solution and lateral flow nucleic acid detection test paper. The method is characterized in that the methylation of the 5 'end of the GP5 gene is identified and amplified based on a PCR method, and the methylation of the 5' end of the GP5 gene is detected by combining a lateral flow technology after an amplification product is labeled. The invention has the advantages that the simple and efficient DNA methylation processing technology and PCR amplification technology are adopted, the nanometer colored latex microspheres are used as visual signals for nucleic acid detection to replace optical signals such as fluorescent probes and the like, complex operation and expensive instruments are not needed, better detection sensitivity and specificity than those of the similar technology or products are realized, the methylation of the 5' end of the GP5 gene is quickly, conveniently, sensitively and economically detected, and the method is suitable for popularization and application and industrialization. |
priorityDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.