http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110241459-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df6a96d0349ae97fbe10332d41282c91 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2019-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0247b81e95e50124ebd55367f5119468 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a029bc5c8eb6c6d371594e5399db30da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_762fe13b5b8acbb82b7a6a9446a934c7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cde66f5731662b54082016216ecdbf4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1bcf563d7308cc607245d8d73a6e6ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e77994786ed4ca7d3492f470f213b388 |
| publicationDate | 2019-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110241459-A |
| titleOfInvention | An immune repertoire method for screening cross-reactivity between samples and independent samples |
| abstract | The invention relates to an immune library method for identifying cross-reactions between samples and independent samples, which is characterized in that: the principle of RACE is used to convert RNA into cDNA when it is reverse-transcribed, and it is performed twice before PCR amplification Pre-amplification, the first pre-amplification makes the 5' end of the original template bring UMI, the second pre-amplification makes the 3' end of the template bring UMI, and then use the product with double-ended UMI As a template for PCR amplification, the upstream and downstream primers used at the same time are all designed with Barcode for labeled PCR amplification. The final product is purified and connected to the sequencing adapter sequence to obtain the final sequencing library. This method realizes the independent sequencing of each sequence. Marking can solve the problem of cross-reaction of similar sequences in the sample during PCR amplification reaction. For samples from different cell receptor sources, only one pair of primers can be used for PCR amplification during PCR amplification, achieving equivalent Amplification, which solves the problem of primer preference caused by multiple primer amplification, and solves the problem of cross-reaction between samples when multiple samples are simultaneously constructed for library construction. |
| priorityDate | 2019-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.