| abstract |
The present invention provides a kind of duck circovirus genetic engineering subunit vaccines and its preparation method and application.The present invention uses sequence nucleic acid molecules as shown in SEQ ID NO:1 or the identical nucleic acid molecule encoding duck circovirus outer capsid structural proteins of 95% or more nucleotide sequence with SEQ ID NO:1, immune composition comprising the duck circovirus outer capsid structural proteins can be used for preparing duck circovirus genetic engineering subunit vaccine, the antigenicity of the vaccine, immunogenicity and function are similar to native protein, expression is higher, immunogenicity is strong, it is not pathogenic to duck, and vaccine of the invention can be prepared by the extensive serum free suspension culture of bioreactor, greatly reduce production of vaccine cost. |