http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110229776-A

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filingDate 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b2d255e3a4e1697bc70bc26c1ef70d6
publicationDate 2019-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110229776-A
titleOfInvention A kind of method for culturing high thermogenic Geobacillus spores
abstract The present invention relates to the field of spore culture of Geobacillus thermophaga. Also disclosed is a method for culturing high thermogenic Geobacillus spores. The method for culturing Geobacillus hyperthermophila spores includes the following steps: S1. High temperature sterilization: take a plurality of petri dishes, rinse the petri dishes with clean water, and place the petri dishes in a pressure steam sterilizer after the washing is completed. , perform high temperature sterilization on the petri dish, the sterilization temperature is 115℃-135℃, and the sterilization time is 15min-40min. After the sterilization is completed, take out the petri dish for use. The method for culturing Bacillus spores with high thermogenicity uses R2A medium as the basic medium, and adds manganese ions and magnesium sulfate to the R2A medium, which significantly increases the number of spores produced, thereby saving time and economy. Cost, the concentration of spores cultivated by this method can reach 1.55×10 8 cfu/mL, which can effectively improve the collection rate of Geobacillus thermophaga spores and achieve more efficient production.
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