http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110218743-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110218743-B |
| titleOfInvention | Construction method of taurine transporter gene knockout rat model based on CRISPR/Cas9 technology |
| abstract | The invention belongs to the technical field of bioengineering, and relates to a construction method of a taurine transporter gene knockout rat model based on CRISPR/Cas9 technology, which comprises the steps of selecting action targets of 2 sgRNAs aiming at the No.5 exon of a tauT gene, synthesizing two pairs of oligonucleotide chains, connecting a pUC57-sgRNA expression vector recovered by BsaI enzyme digestion, constructing to obtain an sgRNA expression vector, obtaining Cas9mRNA and sgRNAs through in vitro transcription, injecting the SgRNA into the male nucleus and cytoplasm of a fertilized egg of an SD rat, implanting into a pseudopregnant rat, and propagating to obtain the tauT gene knockout rat model. |
| priorityDate | 2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.