http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110218735-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-16 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-16 |
| filingDate | 2019-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110218735-B |
| titleOfInvention | Recombinant acetylcholinesterase fixed on surface of bacillus subtilis |
| abstract | The invention relates to a recombinant acetylcholinesterase immobilized on the surface of bacillus subtilis, belonging to the field of molecular biology. Designing and screening a mutation site, carrying out site-directed mutagenesis, then taking bacillus subtilis spore coat protein Cot as a protein carrier for displaying exogenous protein on the surface of spore, and constructing a recombinant spore with the surface displaying a novel acetylcholinesterase protein by using a spore surface displaying technology. According to the invention, through technologies such as molecular docking, molecular dynamics simulation and the like, the acetylcholinesterase gene of drosophila melanogaster is directionally transformed, the heat stability of mutated AChE is obviously increased, and the mutated AChE is fixed on the surface of bacillus subtilis spore, so that the expression, purification and immobilization of enzyme can be completed in one step, the operation process is simple and efficient, the stability of the recombined AChE is improved by using the bacillus subtilis spore, and the bacillus subtilis belongs to probiotics, does not influence human bodies and the environment, and has important significance for improving the utilization efficiency of the bacillus subtilis spore. |
| priorityDate | 2019-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 189.