http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110218712-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6481 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-04004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2016-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110218712-B |
| titleOfInvention | Phospholipase D mutant and application thereof in preparation of phosphatidic acid and phosphatidylserine |
| abstract | The invention belongs to the technical field of enzyme genetic engineering, and particularly relates to a phospholipase D mutant and preparation and application thereof. The technical scheme includes that site-directed mutagenesis is carried out on a wild phospholipase D gene by means of a recombinant DNA technology to obtain a phospholipase D gene with high activity, then the high-activity phospholipase D gene is expressed in a bacillus subtilis expression system and a pichia pastoris expression system (including a pichia pastoris free expression system and a pichia pastoris cell surface display system), a recombinant strain for producing the high-activity phospholipase D is obtained, after expression, the specific enzyme activity of the high-activity phospholipase D is detected to be improved by 38-140% compared with that of the wild phospholipase D, and the highest values of the fermentation enzyme activities of the high-activity phospholipase D in the bacillus subtilis expression system, the pichia pastoris expression system and the pichia pastoris cell surface display system are respectively 36.8U/ml, 48.1U/ml and 140.2U/(g.cell dry weight). |
| priorityDate | 2016-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 627.