| abstract |
The invention discloses a method for preparing nucleotide yeast by enzymatic hydrolysis, which belongs to the field of biochemical industry and biological feed. The specific steps include: continuous fermentation to obtain fermentation broth containing yeast cells, separation of yeast cells, enzymatic hydrolysis of protease to free ribonucleic acid to the outside of the cell, addition of nuclease for directional enzymolysis, and drying of the enzymatic solution to make nucleosides Sour yeast products. The method is simple and easy, and the free nucleotide content of the prepared yeast product can reach more than 11%, which is 2 to 4 times that of similar products at home and abroad (the free nucleotide content of similar products is 2% to 5%). The amino acid nitrogen content is more than 3%, the water solubility is good, and the dissolution rate is more than 90%. The product tastes delicious and has excellent food-attracting and health-care effects on poultry and livestock. |