| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6911
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-549
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate |
2019-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110215522-B |
| titleOfInvention |
A CRISPR/Cas9 delivery system and its preparation method and application |
| abstract |
The present invention provides a CRISPR/Cas9 delivery system and a preparation method and application thereof. The delivery system includes a gold nanocluster inner core, a CRISPR/Cas9 gene editing system attached to the gold nanocluster, and a liposome shell; the CRISPR /Cas9 gene editing system includes sgRNA and Cas9 protein; the Cas9 protein is linked with a nuclear localization signal peptide. The delivery system of the present invention has triple targeting, realizes efficient, specific and nuclear targeted delivery of CRISPR/Cas9, and can effectively reduce the level of LDL-C and reduce the risk of cardiovascular disease by precisely editing the Pcsk9 gene. |
| priorityDate |
2019-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |