http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110208348-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3277 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110208348-B |
| titleOfInvention | Lung cancer detection kit using Nafion as initiator through electrochemical-mediated atom transfer radical polymerization reaction |
| abstract | The invention discloses a lung cancer detection kit by using Nafion as an initiator through an electrochemical mediated atom transfer radical polymerization reaction, which comprises a gold electrode, a PNA solution, [ Fe (CN) ] 6 )] 3‑/4‑ Solution, MCH solution, ZrOCl 2 Solution, Nafion solution, eATRP mixed solution and LiClO 4 And (3) solution. The method comprises the steps of firstly fixing a PNA probe on the surface of a gold electrode through an Au-S bond, then soaking the electrode in MCH to seal the residual active sites, and then dropwise adding a tDNA solution on the electrode plate to hybridize with the PNA to form a PNA/DNA heterotype double chain. Then soaking the hybridized electrode in ZrClO 4 Formation of PNA/MCH/tDNA/Zr in solution 4+ Active probe, sulfonic acid group and Zr on initiator Nafion 4+ Binding, Nafion was attached to the probe. Finally, FMMA is used as a monomer, and can be coordinated and connected with the probe through an eATRP reaction to obtain a polymer chain. The method has higher sensitivity, selectivity, anti-interference performance and accuracy on the detection of the CYFRA21-1 DNA. |
| priorityDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.