http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110205313-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2448 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2019-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110205313-B |
| titleOfInvention | Expression and purification method of aspergillus awamori beta-1, 3-1, 4-glucanase |
| abstract | The invention discloses an expression and purification method of aspergillus awamori beta-1, 3-1, 4-glucanase. The invention provides a method for producing beta-1, 3-1, 4-glucanase, which comprises the steps of introducing an aspergillus awamori beta-1, 3-1, 4-glucanase coding gene into a receptor yeast; culturing the recombinant yeast in a BSM culture medium, and performing a glycerol feeding fermentation stage, a methanol feeding induction expression stage and a mixed feeding induction expression stage when the glycerol is completely consumed. The engineering bacteria of the invention are fermented at high density, and the enzyme activity of the fermentation liquor can reach 159500U/mL (the protein content is 31.7 mg/mL). The optimum pH value of the beta-1, 3-1, 4-glucanase is 5.0, and the beta-1, 3-1, 4-glucanase is kept stable at the pH value of 2.0-9.0; the optimum temperature is 55 ℃, and the high enzyme activity is kept below 60 ℃; the substrate specificity is special. The invention has application value in the industries of food, feed and the like. |
| priorityDate | 2019-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 263.