http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110205285-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_318d3ae9e6b87c6a6b9bedb644827ad0 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
filingDate | 2019-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3884c220f1587e13575a34d13a46e734 |
publicationDate | 2019-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110205285-A |
titleOfInvention | A method for preparing tissue material enriched with autologous stem cells to fill depressions |
abstract | The invention relates to the field of stem cells, and provides a method for preparing a tissue material enriched with autologous stem cells to fill depressions, which is used to solve the problem that tissue filling in depressions in the body is prone to produce rejection reactions. The present invention provides a method for preparing tissue material enriched with autologous stem cells to fill depressions, comprising: S1. extracting autologous adipose mesenchymal stem cells; S2. expanding the mesenchymal stem cells and placing them in a differentiation medium to obtain adipocytes , prepared into an adipocyte fluid, the adipocyte fluid includes adipocytes and a small amount of undifferentiated stem cells, and the differentiation medium includes glucose 15-25 mM, nano ferric oxide particles 60-500 μg/mL, and insulin 15-25 nM , penicillin 50~70U/L, streptomycin 90~110U/L, and the balance is L-DMEM medium; S3. Mix adipocyte fluid with type I collagen to obtain tissue material. Through the differentiation of adipose-derived mesenchymal stem cells, a sufficient amount of adipose tissue can be obtained for filling. At the same time, the obtained fat cells are highly active and relatively complete, and the success rate of the filling operation is high. |
priorityDate | 2019-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 150.