http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110199872-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2019-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110199872-B |
titleOfInvention | Tissue culture and rapid propagation method for stem tips of anthurium andraeanum with short disinfection time |
abstract | The invention discloses a tissue culture and rapid propagation method of a stem tip of a white palm with short disinfection time. The method comprises the following steps: 1) selecting and disinfecting explants; 2) culturing stem tips; 3) inducing cluster buds; 4) and (5) proliferating the cluster buds, and rooting and transplanting. According to the invention, the stem tip with 1-2 leaf primordia and a diameter of 1-2 mm is taken as an explant, so that the disinfection time of 0.1% mercuric chloride can be greatly shortened, the damage of heavy metals to plants is reduced to the maximum extent, the stem tip after disinfection completely survives, the growth and recovery are fast, and the variation risk caused by heavy metal toxicity is reduced. And (3) culturing and proliferating by using cytokinin (0.6-1 mg/L6-BA) with relatively low concentration to obtain higher proliferation multiple, reduce the mutation probability in the proliferation process and improve the quality of the seedlings. Transplanting the rooted plants, wherein the survival rate is more than 99%. |
priorityDate | 2019-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.