http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110195098-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a1575f4c5fd38c0327b4f0e80ce4019 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-112 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc5808621a18f888678fe7b41fc5a733 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2bdc8de8fd7b2b974515d68de1231ed |
| publicationDate | 2019-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110195098-A |
| titleOfInvention | Probe set, kit and application thereof for detecting BRAF-related fusion genes |
| abstract | The invention discloses a probe set for detecting BRAF-related fusion genes, including probe pairs for detecting BRAF/KIAA1549, BRAF/SRGAP3, BRAF/FAM131B, and BRAF/AKAP9 fusion genes, and one of the probe pairs is The needle targets the BRAF gene, and the other probe targets one of KIAA1549, SRGAP3, FAM131B, and AKAP9 genes, and each probe of the probe set is probe-labeled with phi29 DNA polymerase. The invention also discloses a kit for detecting BRAF-related fusion genes, comprising the above-mentioned probe set and hybridization buffer, wherein the hybridization buffer contains dimethyl sulfoxide. In the present invention, by screening the optimal BRAF gene and related partner gene fusion gene detection probes and their combinations, the FISH method is used for fusion gene detection, and the signal counting line is accurate and fast, and the repeatability of the results is good; in the present invention, for the first time, the The phi29 DNA polymerase is used for probe labeling, and the phi29 DNA random primer labeling method is established; combined with fine component adjustment, the labeling effect is better than the currently commonly used gap translation method. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112029832-A |
| priorityDate | 2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 96.