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filingDate 2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110184386-A
titleOfInvention RT-LAMP detection primer for detecting ANRSV and its application, detection reagent and detection method
abstract The invention relates to the technical field of biotechnology and microorganism detection, in particular to RT-LAMP detection primers for detecting ANRSV and applications, detection reagents and detection methods thereof. The sequences of the detection primers are as follows: outer primer ANRSV-F3, outer primer ANRSV-B3, inner primer ANRSV-FIP, inner primer ANRSV-BIP. The areca necrosis ring spot virus (ANRSV) RT-LAMP detection system and detection method of the present invention designs 4 primers with high specificity and sensitivity for 6 regions of the virus gene, and completes the reverse transcription and amplification steps in one step, and the false positive rate Low cost, fast and efficient, and the results can be determined by observing the color change with the naked eye, without steps such as electrophoresis and ultraviolet observation, without the use of thermal cyclers and other instruments, low cost, simple operation, simple identification, suitable for basic laboratory and field testing .
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