| abstract |
The invention discloses a method for screening the core region of banana aquaporin gene promoter, which comprises the following steps: control selection, using transgenic Arabidopsis thaliana transformed with CaMV35S promoter as a control; detection of promoter GUS activity, 100mM, 200mM , 300mM 18% PEG 6000 treatment of 15-day transgenic Arabidopsis seedling leaves and roots were detected for promoter GUS activity; observe the staining situation, from the phenotype point of view, in GUS staining, except for CaMV35S control plants, the GUS activity of each fragment The leaves of the promoter transgenic plants were not stained or stained very lightly, while in the transgenic root system, the staining at the root tip was darker. The result of the present invention identifies the core region of the MaPIP1; 1 promoter, which can further clarify the mechanism of action of the MaPIP1; 1 gene in resisting drought stress. |