| abstract |
Provided is a high-load alkali-resistant protein A magnetic bead, which can maintain its chemical stability in an environment of pH 2-14, and has an ability to bind immunoglobulin IgG greater than 50 mg/mL. Also provided is a method for purifying and/or detecting immunoglobulins, comprising the step of contacting a sample containing immunoglobulins with the high-capacity alkali-resistant protein A magnetic beads. The alkali-resistant protein A magnetic beads provided above can realize rapid purification of immunoglobulin, save about 80% of processing time, and reduce the overall purification cost by 50%. In addition, the alkali-resistant protein A magnetic beads have high alkali resistance, and the alkaline method of in-situ cleaning can be used to regenerate the used magnetic beads. The fast magnetic response and good dispersibility of the magnetic beads can quickly realize the enrichment, washing and elution of the magnetic beads, which is convenient for automatic high-throughput and large-sample purification. |