http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110174514-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2019-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110174514-B |
| titleOfInvention | Streptomyces secondary metabolism efficient promoter mining method based on proteomics and application |
| abstract | The invention discloses a streptomycete secondary metabolism efficient promoter mining method based on proteomics and application thereof. By comparing and analyzing the two-dimensional electrophoresis result of the streptomycete protein, through mass spectrum identification and analysis, genes with obviously improved expression level in secondary metabolism are screened, and the promoter regions of the genes are amplified to be used as alternative promoters. The strong promoter ermE p is used as a reference, the egfp reporter gene is used for characterizing the activity of the alternative promoter, and the relative high activity of the promoter in secondary metabolism is confirmed. In addition, compared with ermE × p, the promoter obtained by screening is applied to highly express the positive regulation factor of the natamycin pathway specificity of the Streptomyces chattanaca, so that the natamycin yield is improved by 20%. The method can be applied to high-yield modification of natural products of streptomycete and is used for regulation and control of the expression of secondary metabolic genes of the streptomycete. |
| priorityDate | 2019-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.