http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110174456-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-447 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 |
| filingDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110174456-B |
| titleOfInvention | Method for measuring lung surfactant protein and application thereof |
| abstract | The invention relates to a method for measuring lung surfactant protein and application thereof, in particular to an electrophoresis technology and a method for directly measuring lung surfactant related protein SP-A, SP-B, SP-C, SP-D in samples such as a lung surfactant preparation (or a lung function research sample) of a mammal, a starting material thereof, a preparation process intermediate and the like without separation preparation or immunoassay technologies such as antibody antigen and the like, wherein an ionic surfactant (SDS) and a strong reducing agent (dithiothreitol) are used for treating the sample by a unique method such as high-temperature and high-speed centrifugation, a proper preformed gel is selected, and clear strips are obtained by electrophoretic separation and are identified and determined to be SP-A, SP-B, SP-C, SP-D respectively. The method does not need to separate the pulmonary surfactant-associated protein from the phospholipid, has short test period and simple and controllable flow, avoids using high-pollution reagents, avoids losing protein components, has more visual and reliable results, and improves the accuracy and precision of the measurement result. |
| priorityDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 386.