| abstract |
The invention discloses a method for producing Ganoderma lucidum triterpenes by liquid submerged fermentation. Cultivate at 25-30°C and 100-180r/min for 8-12 days to obtain a seed culture solution; (2) insert the cultured seed culture solution into the fermentation medium according to the inoculation amount of 8vol%-12vol%, and inoculate After culturing at 25-30°C and 100-180r/min for 7-10 days, adding 0.8g/L-1.5g/L of sodium cholate during the fermentation and culturing, obtaining Ganoderma lucidum mycelium after fermentation, and extracting bacteria Ganoderma lucidum triterpenes in silk. The method of the invention is simple and effective in process, has application value in industrial production, can successfully achieve the purpose of obviously increasing the content of the expression product, shorten the fermentation period, improve the production efficiency, and has the advantages of optimizing the fermentation process of other fungal mycelium. Certain guidance and inspiration. |