http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110158157-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110158157-B |
| titleOfInvention | Method for synthesizing DNA library with fixed length and specific terminal sequence based on template material |
| abstract | The invention relates to the field of biology, and particularly provides a method for synthesizing a DNA library with fixed length and specific terminal sequence based on template materials, which comprises the following steps: obtaining fragmented double-stranded DNA; DNA end repair and 3' end tailing; linkage of the 3' Biotin modified a1 linker; purifying a connection product; DNA is fixed on a solid phase with streptavidin; denaturing the double-stranded DNA into single strands; hybridizing the primer 1; extending bases with dNTPs having reversible chemical modification at the 3' hydroxyl of a deoxyribose group; recovering the hydroxyl at the 3' end of the pentose; a fixed length DNA extension; flattening the 5' end; selecting a terminal sequence; removing a terminal sequence; ligation of linkers for molecular cloning. The method can conveniently construct the region fixed length and the specific terminal sequence library which have no reference genome and high sequence variability, and greatly reduces the construction cost of the library. |
| priorityDate | 2018-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 184.