http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110157749-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-13003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-32 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110157749-B |
| titleOfInvention | Method for synthesizing MK-7 by using bacillus subtilis group response regulation and control system |
| abstract | The invention discloses a method for synthesizing MK-7 by using a bacillus subtilis group response regulation and control system, belonging to the field of genetic engineering. The invention carries out truncation mutation on the phosphokinase KinAAnd use of a constitutive promoter P veg Expression, enabling the cell to produce a spore signal in a nutrient rich medium. The Spo0A system which is regulated by the response of the Phr60-Rap60 population is obtained by applying the method, and the high-efficiency synthesis of MK-7 is realized by dynamically regulating the synthesis way of MK-7, without additional inducer, and the dynamic balance between the high-efficiency synthesis of a target product and the growth of cells is realized. The yields of MK-7 produced by shake flask fermentation of finally obtained Bacillus subtilis recombinant bacteria BS19 and BS20 are respectively as high as 170mg/L and 360mg/L, which are 17.89 times and 37.89 times of Bacillus subtilis 168. |
| priorityDate | 2019-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1151.