http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110146578-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3277
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327
filingDate 2019-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110146578-B
titleOfInvention Method for detecting cholesterol based on RGO-CS-Fc/Pt NPs nano composite material
abstract The invention discloses a method for detecting cholesterol based on RGO-CS-Fc/Pt NPs nano composite material, which comprises the steps of firstly, screen printing an electrode on the basis of activation treatment, forming a layer of gold nanoparticles on the surface of the electrode by an electrodeposition method, modifying RGO-CS-Fc to the surface of the electrode, then, enabling platinum ions to be electrically reduced on the surface of the electrode by a constant potential deposition method to form Pt NPs, then, utilizing the good biocompatibility, high specific surface area, high electron transfer efficiency and high enzyme load characteristic of the RGO-CS-Fc to adsorb a large amount of cholesterol enzyme to construct an enzyme electrode, combining the catalytic amplification effects of the cholesterol enzyme and the Pt NPs, decomposing the cholesterol and generating H 2 O 2 Finally according to the detection H 2 O 2 The redox voltammetry current value is used for drawing a working curve, and high-sensitivity detection of cholesterol is realized.
priorityDate 2019-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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