http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110144318-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01099 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
filingDate | 2019-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110144318-B |
titleOfInvention | Pigment-free low-molecular-weight welan gum production strain and construction method and application thereof |
abstract | The invention discloses a pigment-free low-molecular-weight welan gum production strain, which takes Sphingomonas sp.HT-1 (the preservation number is CCTCC NO: M2012062) as an initial strain, and respectively constructs a pigment-deficient Sphingomonas gene engineering bacterium WG-1 for removing an encapsulation structure, a pigment-and-degrading enzyme gene-deficient Sphingomonas gene engineering bacterium WG-2 and a pigment-and-degrading enzyme gene-deficient Sphingomonas gene engineering bacterium WG-3 for removing an encapsulation structure. The yield of welan gum of the sphingomonas sp WG-2 strain is improved by 10-30 percent compared with that of the original strain, reaches 35-45 g/L, and the molecular weight is not obviously changed (Mn: 10000-20000 kDa); the low molecular weight welan gum (Mn: 500-1000 kDa) is produced by fermenting sphingomonas sp WG-1 and WG-3 strains, and the WG-3 yield of the de-encapsulation structure is improved by 20-30% compared with the WG-1 strain, and reaches 20-25 g/L. The constructed strain can obtain welan gum products with different molecular weight ranges, and the fermentation level of welan gum is obviously improved. |
priorityDate | 2019-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.