http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110133170-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8831 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
filingDate | 2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110133170-B |
titleOfInvention | Protein detection method for complex sample |
abstract | The invention provides a protein detection method for a complex sample, which comprises the following steps: carrying out chromatographic separation and mass spectrum scanning on the complex sample to acquire mass spectrum data, and during the mass spectrum scanning and acquiring the mass spectrum data, adopting a DDA mode to scan and acquire and insert PRM mode scanning and acquisition; and carrying out qualitative and quantitative analysis on the protein in the mass spectrum data to obtain the expression information of the full-spectrum protein of the complex sample. By adjusting the acquisition modes of the two existing mass spectrum data, the PRM acquisition mode is inserted in the DDA acquisition process to more accurately quantify the target protein, and the full-spectrum protein expression condition of the sample and more accurate quantification of the target protein are obtained within a shorter time range. The method has the advantages of high throughput of full-spectrum coverage of protein in a DDA acquisition mode and high sensitivity and accuracy of targeted quantification in a PRM acquisition mode, and is simple to operate, less in time consumption and short in experiment period. |
priorityDate | 2019-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 632.